k ras2a  (Santa Cruz Biotechnology)

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 1. In vitro translation of pKras2A produces a 21-kDa pro- tein. This autoradiograph of a gel containing the protein product of an in vitro translation reaction shows that pKras2A but not a negative control (control (CON) no plasmid) produces a protein of a size consist- ent with full-length K-Ras2A (;21 kDa).

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques: In Vitro, Autoradiography, Negative Control, Control, Plasmid Preparation

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 2. K-Ras2A is an aldosterone-induced protein. A, the left lane (control (CON)) of this Western blot probed with anti-K-Ras2A antibody contains protein from A6 cells not treated with aldosterone that was immunoprecipitated with anti-K-Ras antibody. The right lane (ALDO) contains protein immunoprecipitated from cells treated with aldosterone for 4 h. B, This graph summarizes six such experiments. All paired immunoprecipitants (lines) were performed on the same day and on lysates that contained the same amount of starting, total protein.

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques: Control, Western Blot, Immunoprecipitation

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 3. K-Ras2A is necessary for aldosterone-stimulated Na1

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques:

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 5. Overexpression of K-Ras2A in A6 cells increases Na1

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques: Over Expression

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 7. Aldosterone activates (phosphorylates) MEK 1/2 via K-Ras2A signaling. A these typical Western blots show that cells treated with aldosterone have more protein immunoreactive with anti- phospho-MEK 1/2 antibody compared with cells treated with vehicle (control (CON)). Each lane contains similar amounts of total protein. The time of aldosterone or vehicle treatment is indicated. Note, merely washing cells with vehicle sometimes (as in this figure) appeared to slightly increase phospho-MEK levels; however, this observation was not consistent (observed ;50% of the time). This inconsistent and slight increase in phospho-MEK upon washing results in only a little under- estimation of MEK activation by aldosterone. B, this typical Western blot shows that aldosterone activates MEK in sense (SENSE) but not antisense (ANTI) K-ras2A-treated cells. The top blots were probed with anti-phospho-MEK, and the bottom blots were probed with anti-MEK antibody. All lanes contained similar amounts of total protein.

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques: Western Blot, Control, Activation Assay

Journal: The Journal of biological chemistry

Article Title: Regulation of Na(+) reabsorption by the aldosterone-induced small G protein K-Ras2A.

doi: 10.1074/jbc.274.50.35449

Figure Lengend Snippet: FIG. 8. A possible model of aldosterone signal transduction. Our results support the idea that K-Ras2A synthesis in response to aldosterone is limiting in some instances for regulation of Na1 trans- port by this steroid. Note that the K-Ras2A and methylation signaling pathways, both of which are initiated by aldosterone, converge prior to activation of ENaC. However, other pathways not tested in this study also are likely to be involved in steroid-induction of Na1 reabsorption.

Article Snippet: The polyclonal anti-K-Ras2A antibody (from Santa Cruz) was created against an epitope found only in the A splice variant of K-Ras: the A and B variants diverge at the C terminus between amino acids 165–184.

Techniques: Transduction, Methylation, Protein-Protein interactions, Activation Assay